ZAP70 antibody (pTyr319)

Catalog No. ABIN221118

The Rabbit Polyclonal anti-ZAP70 antibody (ABIN221118) specifically detects ZAP70 in WB, FACS, IF, IP and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for ZAP70 antibody (pTyr319) (ABIN221118)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ZAP70 Antibody

Binding Specificity: pTyr319

Specificity: Detects endogenous levels of human Zap-70 only when phosphorylated at Tyr319. Cross-reacts with endogenous levels of Syk when phosphorylated at Tyr352. Species cross-reactivity: Human and mouse based on 100 % sequence homology.

Purification: Protein A purified

Immunogen: Synthetic phospho-peptide (KLH coupled) corresponding to residues surrounding Tyr319 of human Zap-70.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: Flo (1:25), ICC, IF, IP (1:100), WB (1:1000)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Flow Cytometry. Western Blot: 1:1000. Immunoprecipitation: 1:100. Immunocytochemistry (for IF): 1:100. Flow Cytometry: 1:25.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background: Name/Gene ID: ZAP70
Subfamily: Syk/ZAP70
Family: Protein Kinase

Synonyms: ZAP70, 70 kDa zeta-associated protein, SRK, STCD, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70, TZK, ZAP-70, Zeta-associated protein 70

Gene ID: 7535

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway