Prohibitin antibody

Catalog No. ABIN221967

This anti-Prohibitin antibody is a Mouse Monoclonal antibody detecting Prohibitin in WB, IHC, IF, IHC (p) and IP. Suitable for Human, Mouse, Rat, Pig and Chicken.

Quick Overview for Prohibitin antibody (ABIN221967)

Target: Prohibitin (PHB)

Reactivity: Human, Mouse, Rat, Pig, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prohibitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-Prohibitin Antibody

Specificity: Recognizes rat Prohibitin at 30kD. Species cross-reactivity: Human, porcine, mouse and chicken.

Purification: Protein G purified

Immunogen: Purified recombinant rat prohibitin protein. Cellular Localization: Cytoplasmic.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IF, IHC, IHC-P, IP, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation: 2 μg/mg protein lysate., (Protein G). Native and denatured. Western Blot: 0.5-1 μg/mL for 2 hours at RT. Immunohistochemistry (formalin/paraffin): 2-4 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: MAD109, or PC12 cells, heart.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: PHB / Prohibitin

Background: Name/Gene ID: PHB

Synonyms: PHB, PHB1, Prohibitin

Gene ID: 5245

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling