Prohibitin antibody

Catalog No. ABIN221967

The Mouse Monoclonal anti-Prohibitin antibody (ABIN221967) specifically detects Prohibitin in WB, IHC, IF, IP and IHC (p). The antibody is reactive with Human, Mouse, Rat, Pig and Chicken samples.

Quick Overview for Prohibitin antibody (ABIN221967)

Target: Prohibitin (PHB)

Reactivity: Human, Mouse, Rat, Pig, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prohibitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Prohibitin Antibody

Specificity: Recognizes rat Prohibitin at 30kD. Species cross-reactivity: Human, porcine, mouse and chicken.

Purification: Protein G purified

Immunogen: Purified recombinant rat prohibitin protein. Cellular Localization: Cytoplasmic.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IF, IHC, IHC-P, IP, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation: 2 μg/mg protein lysate., (Protein G). Native and denatured. Western Blot: 0.5-1 μg/mL for 2 hours at RT. Immunohistochemistry (formalin/paraffin): 2-4 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: MAD109, or PC12 cells, heart.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: PHB / Prohibitin

Background: Name/Gene ID: PHB

Synonyms: PHB, PHB1, Prohibitin

Gene ID: 5245

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling