Serotonin Receptor 5A antibody (AA 17-34)

Catalog No. ABIN222307

This Rabbit Polyclonal antibody specifically detects Serotonin Receptor 5A in WB, IF and IHC. It exhibits reactivity toward Mouse and Rat.

Quick Overview for Serotonin Receptor 5A antibody (AA 17-34) (ABIN222307)

Target: Serotonin Receptor 5A (HTR5A)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 5A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-Serotonin Receptor 5A Antibody

Binding Specificity: AA 17-34

Specificity: Recognizes Serotonin Receptor 5A. Species cross-reactivity: Mouse and rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide sequence corresponding to aa17-34 of the rat 5-HT5A receptor.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF, IHC (1:100 - 1:300), WB (1:100 - 1:250)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:100-1:250. Immunohistochemistry: 1:100-1:300 in PBS/0.03 % Triton X-100 - Bn/Av-HRP Technique. Control Tissue: The antiserum demonstrates positive labeling of rat cortex and hippocampus. Perfusion Fixation: Fixative-4 % paraformaldehyde in 0.1 M Phosphate buffer,  pH 7.4, 500 mL over approximately 20 minutes. Post Fixation: 4 % paraformaldehyde in 0.1 M Phosphate buffer,  pH 7.4, 500 mL over approximately 20 minutes. Post Fixation -1.5 hour at 4? C. in 4 % paraformaldehyde in 0.1 M phosphate buffer,  pH 7.4. Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.10.3 %) may be used in conjunction with paraformaldehyde. Sections: 50um vibratome. Absorption control: Synthetic rat 5HT 5A receptor. Incubation on Tissue: 16-24 hours at 4?C.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Serotonin Receptor 5A

Target: Serotonin Receptor 5A (HTR5A)

Alternative Name: HTR5A / 5-HT5A Receptor

Background: Name/Gene ID: HTR5A
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR5A, 5-HT-5, 5-HT-5A, 5-HT5A, 5-HT5a receptor, 5-hydroxytryptamine5a, Serotonin 5-HT5a receptor, Serotonin 5a receptor, Serotonin receptor 5A

Gene ID: 3361

UniProt: P47898

Pathways: JAK-STAT Signaling