HTR7 antibody (AA 8-23)

Catalog No. ABIN222308

The Rabbit Polyclonal anti-HTR7 antibody has been validated for WB, IF and IHC. It is suitable to detect HTR7 in samples from Rat and Mouse.

Quick Overview for HTR7 antibody (AA 8-23) (ABIN222308)

Target: HTR7 (5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTR7 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-HTR7 Antibody

Binding Specificity: AA 8-23

Specificity: Recognizes Serotonin Receptor 7. Species cross-reactivity: Mouse and rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide sequence corresponding to aa8 - 23 of the rat 5-HT7 receptor.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF, IHC (1:100 - 1:300), WB (1:100 - 1:250)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:100-1:250. Immunohistochemistry: 1:100-1:300 in PBS - Bn/Av-HRP Technique. Control Tissue: The antiserum demonstrates positive labeling of rat cortex and. hippocampus. Perfusion Fixation: Fixative-4 % paraformaldehyde in 0.1 M Phosphate buffer,  pH 7.4, 500 mL over approximately 20 minutes. Post Fixation: 4 % paraformaldehyde in 0.1 M Phosphate buffer,  pH 7.4, 500 mL over approximately 20 minutes. Post Fixation-1.5 hour at 4? C. in 4 % paraformaldehyde in 0.1 M phosphate buffer,  pH 7.4. Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.10.3 %) may be used in conjunction with paraformaldehyde. Sections: 50um vibratome. Antibody dilution: 1:100-1:300 in PBS-Bn-SA/HRP Technique. Absorption control: Synthetic rat 5HT 7 receptor. Incubation on Tissue: 16-24 hours at 4?C.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Reconstitute in 100 μL of Sterile distilled water.

Concentration: Lot specific

Buffer: Supplied as a liquid

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HTR7

Target: HTR7 (5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7))

Alternative Name: HTR7 / 5-HT7 Receptor

Background: Name/Gene ID: HTR7
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR7, 5-HT7, 5-HT7 receptor, 5-HT-7, 5-hydroxytryptamine 7 receptor, 5HT7, 5-HT-X, 5-hydroxytryptamine receptor 7, 5HT7 Receptor, HT7, Serotonin 5-HT7 receptor, Serotonin receptor 7

Gene ID: 3363

UniProt: P34969

Pathways: JAK-STAT Signaling