Synaptophysin antibody (C-Term)

Catalog No. ABIN222522

This anti-Synaptophysin antibody is a Rabbit Polyclonal antibody detecting Synaptophysin in WB, IHC and IHC (p). Suitable for Human and Rat.

Quick Overview for Synaptophysin antibody (C-Term) (ABIN222522)

Target: Synaptophysin (SYP)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptophysin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Synaptophysin Antibody

Binding Specificity: C-Term

Specificity: Reacts with neuroendocrine neoplasms of neural as well as epithelial types. In combination with anti-chromogranin A and anti-NSE, very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms. Species cross-reactivity: Human and rat.

Purification: Protein A purified

Immunogen: Synthetic peptide from C-terminus of human synaptophysin.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB (5 - 10 μg/mL)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 5-10 μg/mL for 2 hours at RT. Immunohistochemistry (formalin/paraffin): 5-10 μg/mL for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: IMR-5 cells. Pancreas or Pheochromocytoma or GI tumor.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Synaptophysin

Target: Synaptophysin (SYP)

Alternative Name: SYP / Synaptophysin

Background: Name/Gene ID: SYP

Synonyms: SYP, MRX, Synaptophysin, MRXSYP

Gene ID: 6855

UniProt: P08247

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity