TIMP1 antibody

Catalog No. ABIN222635

The Mouse Monoclonal anti-TIMP1 antibody is suitable to detect TIMP1 in samples from Human. It has been validated for WB, IHC and IHC (p).

Quick Overview for TIMP1 antibody (ABIN222635)

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIMP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TIMP1 Antibody

Specificity: Recognizes human Tissue Inhibitor of Metalloproteinases 1 (TIMP1).

Purification: Protein G purified

Immunogen: Recombinant human TIMP-1. MW of Antigen: 28.5kD. Cellular Localization: Cytoplasmic.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1-2 μg/mL for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1-2 μg/mL for 30 min at RT. Staining of formalin-fixed tissues is improved by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Conditioned, serum-free medium from (TPA-treated) human fetal lung cell line HFL-1. Bladder, breast and ovarian carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for TIMP1

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Alternative Name: TIMP1

Background: Name/Gene ID: TIMP1

Synonyms: TIMP1, CLGI, EPA, HCI, Metalloproteinase inhibitor 1, TIMP-1, Collagenase inhibitor

Gene ID: 7076

UniProt: P01033