Vimentin antibody
Quick Overview for Vimentin antibody (ABIN222821)
Target
See all Vimentin (VIM) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Specificity
- Recognizes Vimentin (57-60kD). Species cross-reactivity: Human, monkey, bovine, equine, porcine, rabbit, canine, feline, rat, mouse, hamster, gerbil and chicken.
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Purification
- Protein G purified
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Immunogen
- Vimentin from porcine eye lens. Cellular Localization: Cytoplasmic.
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Isotype
- IgG1 kappa
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Application Notes
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Approved: IHC, IHC-P
Usage: Suitable for use in Immunohistochemistry. Immunohistochemistry (Formalin/paraffin): 1:50 for 20 min at RT using UltraVision LP Systems. Requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Positive control: Sarcomas. -
Comment
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Target Species of Antibody: Pig
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- PBS, pH 7.4, 0.2 % BSA, 0.09 % sodium azide.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- avoid freeze thaw cycles. Store undiluted.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
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- Vimentin (VIM)
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Alternative Name
- Vimentin
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Background
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Name/Gene ID: VIM
Synonyms: VIM, Vimentin -
Gene ID
- 7431
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UniProt
- P08670
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Pathways
- Caspase Cascade in Apoptosis
Target
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