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Vimentin antibody

The Mouse Monoclonal anti-Vimentin antibody has been validated for IHC and IHC (p). It is suitable to detect Vimentin in samples from Human, Mouse, Rat, Pig, Cow, Chicken, Monkey, Dog, Cat, Rabbit, Hamster and Horse.
Catalog No. ABIN222821

Quick Overview for Vimentin antibody (ABIN222821)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

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Human, Mouse, Rat, Pig, Cow, Chicken, Monkey, Dog, Cat, Rabbit, Hamster, Horse

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Vimentin antibody is un-conjugated

Application

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Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Specificity

    Recognizes Vimentin (57-60kD). Species cross-reactivity: Human, monkey, bovine, equine, porcine, rabbit, canine, feline, rat, mouse, hamster, gerbil and chicken.

    Purification

    Protein G purified

    Immunogen

    Vimentin from porcine eye lens. Cellular Localization: Cytoplasmic.

    Isotype

    IgG1 kappa
  • Application Notes

    Approved: IHC, IHC-P

    Usage: Suitable for use in Immunohistochemistry. Immunohistochemistry (Formalin/paraffin): 1:50 for 20 min at RT using UltraVision LP Systems. Requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Positive control: Sarcomas.

    Comment

    Target Species of Antibody: Pig

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    avoid freeze thaw cycles. Store undiluted.

    Storage

    4 °C,-20 °C

    Storage Comment

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
  • Target

    Vimentin (VIM)

    Alternative Name

    Vimentin

    Background

    Name/Gene ID: VIM

    Synonyms: VIM, Vimentin

    Gene ID

    7431

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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