c-MET antibody (pTyr1234, Tyr1235)

Catalog No. ABIN224750

The Rabbit Polyclonal anti-c-MET antibody has been validated for WB and FACS. It is suitable to detect c-MET in samples from Human.

Quick Overview for c-MET antibody (pTyr1234, Tyr1235) (ABIN224750)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-MET antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-c-MET Antibody

Binding Specificity: pTyr1234, Tyr1235

Specificity: Detects endogenous human HGF R/c-MET phosphorylated at Y1234/Y1235.

Purification: Immunoaffinity purified

Immunogen: Phosphopeptide containing human HGF R/c-MET Y1234/1235 sites.

Isotype: IgG

Application Details

Application Notes: Approved: Flo, WB

Usage: Western Blot: An antibody concentration of 0.5 μg/mL is recommended. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 L. Following a 30 minute incubation, cells should be washed in an isotonic phosphate buffer (supplemented with 0.5 % BSA) prior to the addition of a secondary developing reagent. The binding of unlabeled polyclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL solution of a secondary developing reagent such as anti-rabbit IgG conjugated to a fluorochrome. Cells should be washed for a final time prior to flow cytometric analysis.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for c-MET

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: c-Met

Background: Name/Gene ID: MET
Subfamily: HGF Receptor/MET
Family: Protein Kinase

Synonyms: MET, AUTS9, C-Met, HGF/SF receptor, HGFR, Met kinase, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, HGF receptor, RCCP2, Tyrosine-protein kinase Met

Gene ID: 4233

UniProt: P08581

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor