CTNNB1 antibody (N-Term)
Quick Overview for CTNNB1 antibody (N-Term) (ABIN227284)
Target
See all CTNNB1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- N-Term
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Specificity
- Detects a 92kD protein corresponding to the molecular mass of beta-Catenin on SDS-PAGE immunoblots of Hct116 src transformed cells. Similar results were seen in human endothelial, A431, and HeLa cells.
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Purification
- Immunoaffinity purified
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Immunogen
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beta-Catenin synthetic peptide (coupled to carrier protein) corresponding to amino acid residues in the N-terminal region of human beta-Catenin. This human sequence is highly conserved in rat and mouse beta-Catenins.
Type of Immunogen: Synthetic peptide
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Application Notes
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Approved: ELISA (1:2000), IP, WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation or for antigen applications in immunological protocols. Western blotting 1:1000. Membrane was incubated with diluted antibody in 5 % non-fat milk, PBS, 0.04 % Tween 20 for 1hour at room temperature. Immunoprecipitation: 5-10 μL. ELISA: 1:2000. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- PBS, 50 % glycerol, 1 mg/mL BSA, 0.05 % sodium azide.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
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Alternative Name
- CTNNB1 / Beta Catenin
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Background
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Name/Gene ID: CTNNB1
Family: Catenin
Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin -
Gene ID
- 1499
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UniProt
- P35222
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Pathways
- WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
Target
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