Histone H4 antibody (N-Term)
Quick Overview for Histone H4 antibody (N-Term) (ABIN228407)
Target
See all Histone H4 (HISTH4) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- N-Term
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Specificity
- Recognizes endogenous levels of the human histone H4 protein. Does not cross-react with other histones. Species cross-reactivity: Monkey, mouse and rat. Species sequence homology: Drosophila. Note: Adherence to the attached protocol is extremely important.
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Purification
- Immunoaffinity purified
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Immunogen
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Synthetic peptide (KLH coupled) derived from the amino-terminal sequence of human histone H4 (11kD).
Type of Immunogen: Synthetic peptide -
Isotype
- IgG
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Application Notes
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Approved: ELISA, IHC, IHC-P, IP (1:50), WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunohistochemistry (Paraffin): 1:25. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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Preservative
- Without preservative
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Histone H4 (HISTH4)
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Alternative Name
- Histone H4
Target
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