CMA1 antibody

Catalog No. ABIN228828

This anti-CMA1 antibody is a Mouse Monoclonal antibody detecting CMA1 in WB, IHC and IHC (p). Suitable for Human.

Quick Overview for CMA1 antibody (ABIN228828)

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CMA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CMA1 Antibody

Specificity: May be used to detect mast cells in routinely fixed tissues and has been used to study the distribution of mast cells in skin, synovium, lung and heart. Binding of this antibody to chymase is not reduced in the presence of heparin, suggesting that the antibody does not bind to the heparin-binding domain of chymase. Species cross-reactivity: human.

Purification: Protein G purified

Immunogen: Purified human skin chymase.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P, WB

Usage: Western Blot1 (Ab 1-2 μg/mL for 2hrs at RT). Immunohistochemistry (Formalin/paraffin) (Use Ab at 0.06-0.12 μg/mL for 30 min at RT). Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Tonsil. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4.

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CMA1

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Alternative Name: CMA1 / MCT1 / Chymase

Background: Name/Gene ID: CMA1
Subfamily: Serine S1
Family: Protease

Synonyms: CMA1, Chymase, Chymase, heart, Chymase, mast cell, CYH, Mast cell chymase, Mcpt5, MCT1, Mast cell protease 5, Mast cell protease I, Heart chymase, Alpha-chymase, Chymase 1, Chymase 1, mast cell, CYM

Gene ID: 1215

UniProt: P23946

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Carbohydrate Homeostasis