PKC zeta antibody (pThr410, Thr403)

Catalog No. ABIN229184

The Rabbit Polyclonal anti-PKC zeta antibody has been validated for WB, ELISA, IHC and IHC (p). It is suitable to detect PKC zeta in samples from Human, Mouse, Rat and Monkey.

Quick Overview for PKC zeta antibody (pThr410, Thr403) (ABIN229184)

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC zeta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PKC zeta Antibody

Binding Specificity: pThr410, Thr403

Specificity: Detects endogenous human PKC zeta only when phosphorylated at Thr410, or endogenous human PKC lambda only when phosphorylated at Thr403. Does not cross-react with endogenous levels of other PKC isoforms. Species cross-reactivity: monkey, rat and mouse.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr410 of human PKC zeta.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P, WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (paraffin): 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PKC zeta

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Alternative Name: PRKCZ / PKC-Zeta

Background: Name/Gene ID: PRKCZ
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCZ, PKC-ZETA, PKC2, Pkcz, PKCzeta, Protein kinase c zeta, Protein kinase C zeta type, NPKC-zeta, PKC Zeta, Protein kinase C, zeta, Protein kinase Czeta

Gene ID: 5590

UniProt: Q05513

Pathways: NF-kappaB Signaling, RTK Signaling, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling