BID antibody (Cleavage Site)

Catalog No. ABIN230728

This Rabbit Polyclonal antibody specifically detects BID in WB, ELISA and IP. It exhibits reactivity toward Human.

Quick Overview for BID antibody (Cleavage Site) (ABIN230728)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BID antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-BID Antibody

Binding Specificity: Cleavage Site

Specificity: Detects endogenous levels of both the full length (22kD) and cleaved large fragment of human BID (15kD). Species cross-reactivity: Human.

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to residues surrounding the cleavage site of human BID (KLH coupled).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IP (1:200), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5 % BSA, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:200.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BID

Background: Name/Gene ID: BID
Family: Apoptosis

Synonyms: BID, Apoptic death agonist, BID isoform L(2), BID isoform Si6, Bh3, FP497, p22 BID, BID isoform ES(1b), Desmocollin type 4, Human BID coding sequence

Gene ID: 637

UniProt: P55957

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity