ERBB3 antibody

Catalog No. ABIN230900

The Mouse Monoclonal anti-ERBB3 antibody has been validated for WB and IP. It is suitable to detect ERBB3 in samples from Human, Mouse, Rat and Cow.

Quick Overview for ERBB3 antibody (ABIN230900)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Reactivity: Human, Mouse, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ERBB3 Antibody

Specificity: Recognizes c-erbB-3/HER-3 at 185kD. Species cross-reactivity: Human, bovine, rat and mouse.

Purification: Protein A purified

Immunogen: Recombinant rat c-erbB-3/HER-3 oncoprotein expressed in insect cells and purified by metal-chelating chromatography.

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: IP, WB (1 μg/mL)

Usage: Suitable for use in Immunoprecipitation and Western Blot. Immunoprecipitation (Native and denatured): 2 μg/mg protein lysate (Use Protein A). Western Blot: 1 μg/mL for 2 hrs at RT. Positive control: T47D cells. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Alternative Name: HER3 / ERBB3

Background: Name/Gene ID: ERBB3
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: ERBB3, C-erbB-3, C-erbB3, ErbB-3, HER3, p85-sErbB3, p45-sErbB3, MDA-BF-1, ErbB3-S, LCCS2, p180-ErbB3

Gene ID: 2065

UniProt: P21860

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway