ICAM1 antibody (FITC)

Catalog No. ABIN231146

The Mouse Monoclonal anti-ICAM1 antibody has been validated for WB, FACS, IHC, IP, IHC (p) and IHC (fro). It is suitable to detect ICAM1 in samples from Rat.

Quick Overview for ICAM1 antibody (FITC) (ABIN231146)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is conjugated to FITC

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ICAM1 Antibody (FITC)

Specificity: Recognizes the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), a 90kD adhesion molecule belonging to the immunoglobulin superfamily. CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system. Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling. Functionally 1A29 inhibits homotypic aggregation of PHA blasts. XXX recommend the use of MCA773EL for use in functional studies.

Purification: Protein G purified

Immunogen: Rat Ax cells (a HEV derived cell line).

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:1), IHC, IHC-Fr, IHC-P, IP, WB

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: ICAM1 / CD54

Background: Name/Gene ID: ICAM1
Family: ICAM

Synonyms: ICAM1, BB2, CD54 antigen, CD54, ICAM-1, p3.58

Gene ID: 3383

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling