CD35 antibody

Catalog No. ABIN232371

The Mouse Monoclonal anti-CD35 antibody (ABIN232371) specifically detects CD35 in FACS, IHC, WB, IF and IP. The antibody is reactive with Human and Monkey samples.

Quick Overview for CD35 antibody (ABIN232371)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD35 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-CD35 Antibody

Specificity: Recognizes complement receptor 1 (CR1)/CD35 (210-220kD). Specific for a site that is distal from the C3b-binding site. Does not block CR1 activity. Does not cross-react with CR2. Species cross-reactivity: Human, Rhesus Monkey and Baboon.

Purification: Protein G purified

Immunogen: Intact human monocytes.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, IF, IHC (2 - 4 μg/mL), IP, WB

Usage: Suitable for Flow Cytometry, Immunofluorescence, Immunohistochemistry. Not suitable for use in Western Blot and Immunoprecipitation. Other applications. Immunohistochemistry: 2-4 μg/mL. Strongly labels follicular dendritic cells (FDC). Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 15 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CR1 / CD35

Background: Name/Gene ID: CR1
Subfamily: Serine S1
Family: Protease

Synonyms: CR1, C3b/C4b receptor, C3-binding protein, CD35, C3BR, Knops blood group antigen, C4BR, CD35 antigen, Complement receptor type 1, KN

Gene ID: 1378

UniProt: P17927

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity