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CD35 antibody

CR1 Reactivity: Human, Monkey FACS, WB, IHC, IF, IP Host: Mouse Monoclonal unconjugated

Catalog No. ABIN232371

Datasheet as PDF

Target See all CD35 (CR1) Antibodies

CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))
Reactivity
107

26

25

16

16

16

16

4

3
Human, Monkey
Host
66

45

3
Mouse
Compatible Secondaries
Clonality
78

33

3
Monoclonal
Conjugate
50

11

8

7

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD35 antibody is un-conjugated
Application
70

35

31

25

20

20

19

18

17

13

13

11

3

2

2

2

2

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificity

Recognizes complement receptor 1 (CR1)/CD35 (210-220kD). Specific for a site that is distal from the C3b-binding site. Does not block CR1 activity. Does not cross-react with CR2. Species cross-reactivity: Human, Rhesus Monkey and Baboon.

Purification

Protein G purified

Immunogen

Intact human monocytes.

Type of Immunogen: Cells

Isotype

IgG1

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CR1 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal E11 FITC

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CR1 Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

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CR1 Reactivity: Human IHC, ELISA Host: Rabbit Polyclonal unconjugated

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CR1 Reactivity: Human IHC, ELISA Host: Rabbit Monoclonal 9A9 unconjugated Recombinant Antibody

anti-Complement Component Receptor 1 (CD35) (CR1) antibody (PE)
CR1 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal E11 PE

Application Notes

Approved: Flo, IF, IHC (2 - 4 μg/mL), IP, WB

Usage: Suitable for Flow Cytometry, Immunofluorescence, Immunohistochemistry. Not suitable for use in Western Blot and Immunoprecipitation. Other applications. Immunohistochemistry: 2-4 μg/mL. Strongly labels follicular dendritic cells (FDC). Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS,  pH 7.4, 0.2 % BSA, 15 mM sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
Target

CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name

CR1 / CD35 (CR1 Products)

Background

Name/Gene ID: CR1
Subfamily: Serine S1
Family: Protease

Synonyms: CR1, C3b/C4b receptor, C3-binding protein, CD35, C3BR, Knops blood group antigen, C4BR, CD35 antigen, Complement receptor type 1, KN

Gene ID

1378

UniProt

P17927

Pathways

Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity