CDK1 antibody (pThr161)

Catalog No. ABIN232708

This Rabbit Polyclonal antibody specifically detects CDK1 in WB, ELISA, IF, IP and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CDK1 antibody (pThr161) (ABIN232708)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CDK1 Antibody

Binding Specificity: pThr161

Specificity: Detects endogenous levels of cdc2 only when phosphorylated at Thr161. Cross-reacts with endogenous cdk2 phosphorylated at Thr160. Species cross-reactivity: Mouse and rat.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr161 of human cdc2 (KLH coupled).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, IF, IP (1:50), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunofluorescence. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5 % BSA, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunocytochemistry (for IF): 1:200.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: CDK1 / CDC2

Background: Name/Gene ID: CDK1
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK1, CDC28A, CDC2, CDKN1, Cyclin-dependent kinase 1, p34 protein kinase, p34CDC2, CDC2a, Cell cycle controller CDC2, Cell division protein kinase 1, PSTAIR

Gene ID: 983

UniProt: P06493

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA