DBH antibody (AA 40-57)

Catalog No. ABIN232869

This anti-DBH antibody is a Rabbit Polyclonal antibody detecting DBH in WB and IHC. Suitable for Human.

Quick Overview for DBH antibody (AA 40-57) (ABIN232869)

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DBH Antibody

Binding Specificity: AA 40-57

Specificity: Recognizes Dopamine Beta Hydroxylase (DbH) N-terminal. By immunoblot the antibody specifically reacts with a single (or double) band (s) of M. W. 70-75000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE. Species cross-reactivity: By immunoblot: human, monkey (slight), sheep, canine (slight), rat and bovine. Also shown to react with equine ganglia cells.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon (100%) Gorilla (94%) Marmoset (89%) Mouse, Rat, Elephant, Porcine, Bovine (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide, C-SAPRESPLPYHIPLDPEG-amide, cloned from human (DbH) cDNA. Percent identity by BLAST analysis: Human, Gibbon (100%), Gorilla (94%), Marmoset (89%), Mouse, Rat, Elephant, Porcine, Bovine (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC (1:250 - 1:1000), WB (1:250 - 1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:250-1:1000. Immunohistochemistry: 1:250-1:1000. Staining was evaluated in the locus ceruleus of formaldehyde-fixed, cryopreserved human and monkey brain. Showed weak staining on both human and monkey.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Dulbecco's PBS (divalent-deficient), 10 % glycerol, 10 mM HEPES,  pH 7.2, 20 mM Tris-HCl,  pH 7.6, 0.1 % PVP-40, 0.05 % Tween-20, 0.1 % sodium azide, 1 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for DBH

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Alternative Name: DBH/Dopamine Beta Hydroxylase

Background: Name/Gene ID: DBH
Family: Monooxygenase

Synonyms: DBH, Dopamine beta-monooxygenase, DBM, Dopamine beta-hydroxylase

Gene ID: 1621

UniProt: P09172

Pathways: Carbohydrate Homeostasis