EEF2 antibody (N-Term)

Catalog No. ABIN232872

This anti-EEF2 antibody is a Rabbit Polyclonal antibody detecting EEF2 in WB, ELISA, IF and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for EEF2 antibody (N-Term) (ABIN232872)

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-EEF2 Antibody

Binding Specificity: N-Term

Specificity: Detects endogenous levels of total eEF2 independent of phosphorylation (Mr 100kD). Species cross-reactivity: Human, mouse and rat. Species sequence homology: chicken and hamster 100 % .

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (KLH coupled) corresponding to residues mapping at the N-terminus of human eEF2.

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, IF, WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunofluorescence. Western Blot: 1:1000, Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunocytochemistry (for IF): 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for EEF2

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Alternative Name: EEF2 / Elongation Factor 2

Background: Name/Gene ID: EEF2

Synonyms: EEF2, EEF-2, EF-2, Elongation factor 2, Polypeptidyl-tRNA translocase, EF2

Gene ID: 1938

UniProt: P13639

Pathways: AMPK Signaling