FADD antibody (pSer194)

Catalog No. ABIN232979

The Rabbit Polyclonal anti-FADD antibody has been validated for WB, ELISA and ICC. It is suitable to detect FADD in samples from Human.

Quick Overview for FADD antibody (pSer194) (ABIN232979)

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FADD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-FADD Antibody

Binding Specificity: pSer194

Specificity: Detects endogenous levels of human FADD protein only when phosphorylated at Ser194.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser194 of human FADD.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, WB (1:1000)

Usage: Suitable for use in ELISA, Immunocytochemistry and Western Blot. Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunocytochemistry (ABC): 1:200.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FADD

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Alternative Name: FADD

Background: Name/Gene ID: FADD

Synonyms: FADD, GIG3, MORT1, Protein FADD

Gene ID: 8772

UniProt: Q13158

Pathways: Apoptosis, TLR Signaling, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades