GFAP antibody

Catalog No. ABIN233055

The Mouse Monoclonal anti-GFAP antibody (ABIN233055) specifically detects GFAP in WB, IHC, IF and IHC (p). The antibody is reactive with Human, Rat, Pig and Chicken samples.

Quick Overview for GFAP antibody (ABIN233055)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Pig, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GFAP Antibody

Specificity: Stains human astrocytes, glial cells, ependymal cells and their corresponding tumors. Does not stain many types of neural tumors such as neuroblastomas, Schwannomas and extra-CNS tumors. Species cross-reactivity: porcine, rat, chicken.

Purification: Protein G purified

Immunogen: Glial Fibrillary Acidic Protein (51-52kD). Cellular Localization: Cytoplasmic.

Isotype: IgG1

Application Details

Application Notes: Approved: IF, IHC, IHC-P, WB (1 - 2 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Western Blot: 1-2 μg/mL for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1-2 μg/mL for 30 min at RT. Note: No special pretreatment required prior to staining of formalin-fixed, paraffin-embedded sections. Positive control: IMR-5 cells, Brain or astrocytoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: Name/Gene ID: GFAP

Synonyms: GFAP

Gene ID: 2670

UniProt: P14136