PKM2 antibody (Asn491, C-Term) (HRP)

Catalog No. ABIN2336512

This Rabbit Polyclonal antibody specifically detects PKM2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PKM2 antibody (Asn491, C-Term) (HRP) (ABIN2336512)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKM2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Product Details anti-PKM2 Antibody (HRP)

Binding Specificity: Asn491, C-Term

Specificity: Recognizes human PKM2.

Cross-Reactivity: Human, Mouse (Murine)

Cross-Reactivity (Details): Calculated cross reactivity: Hu Mo

Characteristics: PKM2, CT (N491) (Pyruvate Kinase, Isozymes M1/M2, Pyruvate Kinase Muscle Isozyme, Cytosolic Thyroid Hormone-binding Protein, CTHBP, Opa-interacting Protein 3, OIP-3, Pyruvate Kinase 2/3, Thyroid Hormone-binding Protein 1, THBP1, Tumor M2-PK, p58, PKM, OIP3, PK2, PK3) (Azide free) (HRP)

Purification: Purified by Protein G affinity chromatography.

Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 476-505 in the C-terminal region of human PKM2, UniProt Accession #NP_002645.

Isotype: IgG

Application Details

Application Notes: Optimal working conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: PKM2

Background: PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.

NCBI Accession: NP_002645

UniProt: P14618

Pathways: Warburg Effect