PKM2 antibody (C-Term)

Catalog No. ABIN391053

Product Details anti-PKM2 Antibody

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Binding Specificity: AA 476-505, C-Term

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).

Clone: RB3697

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:200. WB: 1:1000. WB: 1:1000. WB: 1:2000. IHC-P: 1:50~1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-PKM2 antibody (ABIN391053)

Kienzl, Sarg, Golderer, Obrist, Werner, Werner-Felmayer, Lindner, Maglione, Schneeberger, Margreiter, Brandacher: "Proteomic profiling of acute cardiac allograft rejection." in: Transplantation, Vol. 88, Issue 4, pp. 553-60, (2009) (PubMed).

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: PKM2 (PKM2 Products)

Background: There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.

Molecular Weight: 57937

Gene ID: 5315

NCBI Accession: NP_001193725, NP_001193726, NP_001193727, NP_001193728, NP_002645, NP_872270, NP_872271

UniProt: P14618

Pathways: Warburg Effect