FKBP8 antibody (Internal Region)

Catalog No. ABIN233735

Supplier Product No.: 200-401-a58

Product Details anti-FKBP8 Antibody

Target: FKBP8 (FK506 Binding Protein 8, 38kDa (FKBP8))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FKBP8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 200-401-a58

Supplier: Rockland

Purpose: FKBP8 Antibody

Cross-Reactivity (Details): This antibody is specific for human FKBP8 protein.

Characteristics: Synonyms: rabbit anti-FKBP8 Antibody, FKBP 8, FKBP-38, 38 kDa FK 506 binding protein homolog antibody, 38 kDa antibody, FK506 binding protein 8 antibody, FKBP 38 antibody

Purification: This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human FKBP8 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This protein A purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 64 kDa in size corresponding to FKBP8 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for FKBP8

Target: FKBP8 (FK506 Binding Protein 8, 38kDa (FKBP8))

Alternative Name: FKBP8 (FKBP8 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). FKBP8 (also known as FK506-binding protein 38) is a member of the immunophilin family that has been implicated to play an important role in apoptosis through its involvement in the mechanism that targets Bcl-2 and Bcl-xL to the outer mitochondrial membrane (OMM). Suppression of endogenous FKBP8 by RNAi or transfection of a mutant FKBP8 missing the transmembrane domain necessary for mitochondrial insertion, resulted in the translocation of Bcl-2 and Bcl-xL from the OMM to the cytosol. It has also been suggested that FKBP8 may play a role in the regulation of apoptosis. Anti-FKBP8 Antibody is useful for researchers interested in HIV, Cancer, and Metabolism of protein research.

Gene ID: 23770, 52630440

UniProt: Q14318

Pathways: Autophagy