SMAD3 antibody (Internal Region)

Catalog No. ABIN233815

Supplier Product No.: 600-401-973

This Rabbit Polyclonal antibody specifically detects SMAD3 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SMAD3 antibody (Internal Region) (ABIN233815)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SMAD3 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-973

Supplier: Rockland

Purpose: SMAD3 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human Smad3 protein.

Characteristics: Synonyms: rabbit anti-SMAD3 antibody, SMAD-3, SMAD 3, mothers against decapentaplegic homolog 3 antibody, MAD homolog 3, Mothers against DPP homolog 3, SMAD family member 3, MADH3, MADH 3, JV15-2

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 50 kDa in size corresponding to Smad3 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:10,000

ELISA Dilution: 1:15,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.06 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-b (transforming growth factor) and activin type I receptor kinase.  These activators exert diverse effects on a wide array of cellular processes.  The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily.  Briefly, activin type I receptor kinase phosphorylates receptor-activated Smads (R-Smads) at the two extreme serines in the C-terminal SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the Bone Morphogenetic Proteins (BMP) pathway.  The phosphorylated R-Smad then translocates into the nucleus, where it regulates transcription of target genes.  Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-b-mediated responses.

Gene ID: 4088, 5174513

UniProt: P84022

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy