c-MET antibody (pTyr1349, pTyr1356)

Catalog No. ABIN233821

Supplier Product No.: 600-401-989

Product Details anti-c-MET Antibody

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Binding Specificity: pTyr1349, pTyr1356

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-MET antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-989

Supplier: Rockland

Purpose: c-Met phospho Y1349 / pY1356 Antibody

Cross-Reactivity (Details): Antibody is specific for human c-Met protein phosphorylated at Y1349 and Y1356.

Characteristics: Synonyms: rabbit anti-c-Met pY1349/pY1356 Antibody, rabbit anti-c-Met dual phosphorylated Antibody, HGF receptor antibody, HGF/SF receptor antibody, HGFR antibody, MET antibody, Met proto oncogene tyrosine kinase antibody, SF receptor, Tyrosine-protein kinase Met, Scatter factor receptor, Proto-oncogene c-Met, Hepatocyte growth factor receptor

Purification: Phospho c-MET Antibody was produced from monospecific antiserum by immunoaffinity chromatography using dual-phospho-peptide coupled to agarose beads followed by cross-adsorption against nonphospho-peptide.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1349 and Y1356 of human c-Met protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 150 kDa in size corresponding to phosphorylated c-Met protein by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with human c-Met pY1349pY1356 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:93,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.01 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Phospho antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for c-MET

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: MET (MET Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-c-MET is the receptor for hepatocyte growth factor (also known as scatter factor, HGF/SF), and belongs to the tyrosine kinase superfamily. Interaction of c-Met with HGF results in autophosphorylation of c-Met at multiple tyrosines.  Phosphorylation of Y1234/1235 in the c-Met kinase domain is critical to kinase activation.  When phosphorylated, Y1349 and Y1356, along with surrounding amino acids, form a unique bidentate docking site for substrates such as Gab1, Grb2, phosphatidylinositol 3-kinase (PI3K) and others.  C-Met mainly uses the Gab1 scaffolding adaptor in its initial step of signal transmission.  Well-characterized downstream signaling pathways that are activated by c-Met include the ERK/MAPK, PI3K-Akt/PKB, Crk-Rap and Rac-Pak pathways, resulting in proliferation and increased cell survival. Anti-Phospho cMET was developed with NCI and is ideal for Cancer Research.

Gene ID: 4233, 42741655

UniProt: P08581

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor