SMAD4 antibody (C-Term)

Catalog No. ABIN233840

Supplier Product No.: 600-401-a41

Product Details anti-SMAD4 Antibody

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-a41

Supplier: Rockland

Purpose: SMAD4 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human SMAD4 protein.

Characteristics: Synonyms: rabbit anti-SMAD4 antibody, SMAD-4, SMAD 4, mothers against decapentaplegic homolog 4 antibody, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4 antibody, SMAD family member 4, DPC 4, DPC4, MADH4, MADH 4

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human SMAD4 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 60 kDa in size corresponding to SMAD4 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:15,000 - 1:60,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4 (SMAD4 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. SMAD4 (also known as Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, deletion target in pancreatic carcinoma 4 and hSMAD4) is a common mediator of signal transduction by TGF-b (transforming growth factor), but is also involved in cancer development and metastases as a tumor suppressor.  SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription.  SMAD4 may form trimers with receptor-regulated SMAD (R-SMAD) and interacts with ATF2, COPS5, DACH1, MSG1, SKI and TRIM33.  In the absence of ligand SMAD4 is found in the cytoplasm, but when complexed with R-SMAD, translocates to the nucleus.   Defects in SMAD4 are a cause of pancreatic carcinoma and juvenile polyposis syndrome (JPS), a syndrome in which patients are at risk for developing gastrointestinal cancers.

Gene ID: 4089, 13603414

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy