ODF2 antibody (pSer796)

Catalog No. ABIN233845

Supplier Product No.: 600-401-a47

Product Details anti-ODF2 Antibody

Target: ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))

Binding Specificity: pSer796

Reactivity: Human, Chimpanzee, Macaque

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ODF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-a47

Supplier: Rockland

Purpose: Cenexin-1 phospho S796 Antibody

Cross-Reactivity (Details): This antibody is specific for human Cenexin-1 protein phosphorylated at S796.

Characteristics: Synonyms: rabbit anti-Cenexin-1 pS796 Antibody, ODF84 antibody, Outer dense fiber of sperm tail 2, Cenexin1 variant 1 antibody, KKT 4 antibody, ODF2, Cenexin 1, Cenexin-1, Outer dense fiber protein 2

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near S796 of human cenexin-1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 93 kDa in size corresponding to phosphorylated Cenexin-1 by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with human Cenexin-1 pS796 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Western Blot Dilution: 1:200 - 1:2,000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ODF2

Target: ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))

Alternative Name: ODF2 (ODF2 Products)

Background: Background: Cenexin-1, also known as ODF2 and outer dense fiber of sperm tails 2, are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation.  Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. Cenexin-1 is one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of the cenexin gene, however, not all isoforms and variants have been fully described.

Gene ID: 4957

NCBI Accession: NP_702915

UniProt: Q5BJF6

Pathways: M Phase