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Antigen | Outer Dense Fiber of Sperm Tails 2 (ODF2) |
Method validated | Western Blotting |
Positive Control | recombinant ODF2-His, bacterially expressed; NIH3T3 total cell lysate |
Notes | Passed, regarding sensitivity and specificity. The antibody detects bacterially expressed ODF2 (see 2)) and the endogenous protein (see 3)) with only moderate background staining in WB. |
Primary Antibody | ABIN2430582 |
Secondary Antibody | anti rabbit IgG (H+L), HRP-linked (Jackson Immuno Research, Code number: 111-035-003, Lot number: 123450) |
Protocol |
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Antigen | Outer Dense Fiber of Sperm Tails 2 (ODF2) |
Method validated | Immunocytochemistry |
Positive Control | mouse anti-γ-tubulin (Sigma-Aldrich, clone GTU-88, T6557, lot number 072M4808) |
Notes | Passed, the antibody detects centrosomal ODF2 in immunocytological preparations with only very low background staining (the green dots are mostly the correct location of ODF2 as they overlap with γ-tubulin spots (in red)). |
Primary Antibody | ABIN2430582 |
Secondary Antibody | MFP488 goat anti-rabbit IgG (H+L) (MoBiTec, #MFP-A1008) |
Protocol |
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Application Notes | Optimal working dilution should be determined by the investigator. |
Restrictions | For Research Use only |
Format | Liquid |
Handling Advice | Avoid freeze / thaw cycles. |
Storage | -20 °C/-80 °C |
Storage Comment | Store at -20°C (regular) and -80°C (long term). |
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