beta Amyloid antibody (C-Term)

Catalog No. ABIN234280

This Mouse Monoclonal antibody specifically detects beta Amyloid in WB, ELISA and ICC. It exhibits reactivity toward Human.

Quick Overview for beta Amyloid antibody (C-Term) (ABIN234280)

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Amyloid antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Clone: BDI350

Product Details anti-beta Amyloid Antibody

Binding Specificity: AA 1-40, C-Term

Specificity: Specifically reacts with the C-terminus of Human beta Amyloid 1-40. Does not cross-react with beta Amyloid 1-42.

Characteristics: MAb to Amyloid Beta (1-40), C-terminal, Monoclonal antibody to Amyloid Beta 1-40, C-terminal, Tissue culture supernatant

Purification: Thiophilic adsorption and size exclusion Chromatography. Tissue culture supernatant

Immunogen: C-terminal peptide conjugated to KLH

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 1 µg/mL HRP/ECL detection. Recommended blocking with casein/tween 20 based buffer. Western blot Analysis Amyloid beta A4 peptides were applied to SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 2- g/mL Q67350M for 1 hour at 15-22-C and developed by ECL (exp. time: 30 sec.). Lane 1: bA4 (1-40) Lane 2: bA4 (1-42) Lane 3: bA4 (1-43) Immunocytochemistry: 1-10 µg/mL ELISA: 0.1 µg/mL Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 1 mL water (15 minutes at room temperature)

Buffer: Lyophilized from 2X PBS, PEG and Sucrose, 0.09 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Store lyophilized product at -20 °C. After reconstitution, aliquot and store at -80 °C. Thawed aliquots may be stored (up to 3 months) at 2-0 °C.

Expiry Date: 3 months

Target Details for beta Amyloid

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Alternative Name: Amyloid Beta

Pathways: Inflammasome