beta-2 Microglobulin antibody

Catalog No. ABIN234374

The Mouse Monoclonal anti-beta-2 Microglobulin antibody has been validated for IHC (f). It is suitable to detect beta-2 Microglobulin in samples from Human.

Quick Overview for beta-2 Microglobulin antibody (ABIN234374)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: B1G6

Product Details anti-beta-2 Microglobulin Antibody

Specificity: Specific for human Beta 2-microglobulin (MW 12 kDa). Does not react with Beta 2-microglobulin from other species. Recognizes the HLA-Beta 2-microglobulin complex, free or associated with the membrane. The exact epitope for the antibody has not been mapped.

Characteristics: MAb to Beta 2-Microglobulin, Monoclonal antibody to Beta 2-Microglobulin

Purification: Purified

Immunogen: Purified human Beta 2-microglobulin

Isotype: IgG2a

Application Details

Application Notes: Purification of HLA antigens associated with Beta 2-microglobulin. Fluorescence microscopy or flow cytometry: 2 µg/5x105 cells/test. Immunohistochemistry: Working dilution: 1:25 - 1:50 (suitable on frozen sections or cell smears) Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 1 mL distilled water.

Buffer: Lyophilized from PBS containing 1 mg/mL BSA.

Preservative: Without preservative

Handling Advice: Avoid multiple freeze/thaw cycles. The addition of 0.1 % (w/v) sodium azide is recommended for storage of the Reconstituted form for up to one month at 2-8 °C.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized product at 2-8 °C. After reconstitution, aliquot and store at -28 °C.

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: Beta 2-Microglobulin

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process