CD63 Gp 53 antibody (FITC)

Catalog No. ABIN234721

Product Details

Target: CD63 Gp 53

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), Indirect Immunofluorescence Assay (IFA)

Cross-Reactivity (Details): The molecular weight of the recognized platelet form of CD63 is 40 to 55kDa. CLBGran/12 reacts with most peripheral blood cells including activated platelets, lymphoid, myeloid and endothelial cells, except for red blood cells and resting T cells. The CD63 antigen is present in many tissues and cell line PHA blasts, K562, U937, KG1a, RAJI. Both surface and cytoplasmotic reactions are reported. CLB Gran/12 has been assigned to the CD63 cluster of differentiation at the IVth International Workshop on Human Leucocyte Differentiation Antigens.

Characteristics: MAb to CD63, Monoclonal antibody to CD63 gp 53 Fluorescein conjugated

Purification: Ion Exchange or affinity Chromatography. Ascites

Immunogen: Human cytochrome B enriched cells

Clone: CLBGran-12

Isotype: IgG1

Application Details

Application Notes: Detection of activated platelets, neutrophils and basophils. Fluorescence microscopy or flow cytometry: 20 µL/106 platelets/test or 20 µL/5 x 105 cells/test Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M Sodium phosphate, 0.141 M Sodium chloride, pH 7.2 containing 2 mg/mL BSA, 0.1 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Storage: 4 °C

Storage Comment: Store (in the dark) at 2-8 °C.

Target Details

Target: CD63 Gp 53