Botulinum Neurotoxin Type B (BoNT/B) (AA 1278-1291), (C-Term) antibody

Catalog No. ABIN234815

The Mouse Monoclonal anti-Botulinum Neurotoxin Type B antibody is suitable to detect Botulinum Neurotoxin Type B in samples from Clostridium botulinum. It has been validated for ELISA, RIA and IFA.

Quick Overview for Botulinum Neurotoxin Type B (BoNT/B) (AA 1278-1291), (C-Term) antibody (ABIN234815)

Target: Botulinum Neurotoxin Type B (BoNT/B)

Reactivity: Clostridium botulinum

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Radioimmunoassay (RIA), Indirect Immunofluorescence Assay (IFA)

Clone: B362M

Product Details

Binding Specificity: AA 1278-1291, C-Term

Specificity: Binds to intact whole C. botulinum Toxin B, amino acids 1278-1291. Has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of C. botulinum Toxin B. Inhibits the entry of C. botulinum Toxin B to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin.

Cross-Reactivity (Details): Cross-reactivity: C. botulinum Toxin B (1278-1291) 100 % C. botulinum Toxin B 100 % C. botulinum Toxin A 0 % C. botulinum Toxin C 0 % C. botulinum Toxin E 0 % C. botulinum Toxin F (1272-1280) 0 %

Characteristics: MAb to C. botulinum Toxin B, Monoclonal antibody to Clostridium botulinum Toxin B (AA 1278-1291)

Purification: Protein A column. Ascites

Immunogen: Synthetic peptide analogue of the carboxyl terminal region of C. botulinum Toxin B (Catalog ABIN573256)

Isotype: IgG

Application Details

Application Notes: Suitable for use in ELISA, IFA and RIA. Suitable for use in an indirect immunocytochemical staining procedure at a dilution of 1:25. Prior incubation of the antibody with synthetic peptide immunogen has been shown to eliminate the inhibition of cellular binding by the antibody. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Without preservative

Handling Advice: Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Storage: -20 °C

Storage Comment: Upon receipt, store at -20 °C.

Target Details

Target: Botulinum Neurotoxin Type B (BoNT/B)

Alternative Name: Clostridium Botulinum Toxin B