Growth Hormone 1 antibody

Catalog No. ABIN235517

The Mouse Monoclonal anti-Growth Hormone 1 antibody has been validated for ELISA and has been independently validated. It is suitable to detect Growth Hormone 1 in samples from Human.

Quick Overview for Growth Hormone 1 antibody (ABIN235517)

Target: Growth Hormone 1 (GH1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Growth Hormone 1 antibody is un-conjugated

Application: ELISA

Clone: 090-10154

Product Details anti-Growth Hormone 1 Antibody

Cross-Reactivity (Details): Cross-reactivity: Synthetic hGH (Somatonorm) 100 % Human Placental Lactogen <0.00 % Human Prolactin <0.00 %

Characteristics: MAb to hGH, Monoclonal antibody to Human Growth Hormone (hGH)

Purification: Protein A Chromatography. Ascites

Sterility: 0.2 μm filtered

Immunogen: hGH isolated from human pituitary gland

Isotype: IgG1

Application Details

Application Notes: Suitable for use in ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Independent Validation (UP)

Validation #102719 (Unfolding Profile)

by: NanoTemper Technologies

No.: #102719

Date: 11/05/2018

Antigen: GH1

Lot Number: H0833.1

Method validated: Unfolding Profile

Positive Control: ABIN235517

Notes:

Passed. ABIN235517 showed a clear unfolding profile with one detected unfolding event with a T_i at 76.3°C. This suggests that the protein is properly folded and functional.

Validation Images

Unfolding profile of ABIN235517. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 76.3°C.

Full Methods

Primary Antibody: ABIN235517

Full Protocol:

• Dilute ABIN235517 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 3.33µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Experimental Notes:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Handling

Format: Liquid

Buffer: 10 mM Phosphate, pH 7.4 containing 150 mM Sodium chloride, 0.1 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Storage: 4 °C/-80 °C

Storage Comment: Short term (up to 7 days) store at 2-8 °C. Long term, aliquot and store at <-48 °C. If aliquoted for long term storage, fill volume should be equal to or greater than 50 % of the nominal fill volume of the vial used.

Expiry Date: 1 week

Target Details for Growth Hormone 1

Target: Growth Hormone 1 (GH1)

Alternative Name: Growth Hormone (hGH)

Pathways: NF-kappaB Signaling, JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process