MAP2 antibody

Catalog No. ABIN235804

This Mouse Monoclonal antibody specifically detects MAP2 in WB and IHC (f). It exhibits reactivity toward Cow.

Quick Overview for MAP2 antibody (ABIN235804)

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: AP20

Product Details anti-MAP2 Antibody

Specificity: Anti-MAP-2 recognizes (Mr 300 kDa) MAP-2 protein from bovine brain. Strongly cross-reacts with MAP-2 from human, rat, mouse and chicken brains. Anti-MAP-2 (Clone AP20) reacts with the high molecular weight forms (2a & 2b) of MAP-2 but not with the low molecular weight form (2c). Reacts with dendrites and cell bodies of neurons.

Characteristics: MAb to MAP-2 (2a + 2b), Monoclonal antibody to Microtubule Associated Protein-2 (MAP-2) (2a + 2b), Cell culture

Purification: R-phycoerythrin has been covalently conjugated to anti-human CD45RO and chromatographically purified to remove unconjugated antibody and dye, while achieving a fluorochrome/protein (F/P) molar ratio between 0.7-1.3. R-phycoerythrin has a maximum absorbance of 565.5nm. Tissue culture

Isotype: IgG1

Application Details

Application Notes: Can be used in protein blotting and immunohistochemistry, frozen tissues only. Anti-MAP-2 may be used in studies of neuron structure in brain tissue. The suggested working dilution for immunohistochemical staining is approximately 1:500. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M PBS, pH 7.2 containing 1 % BSA, 0.01 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Short-term (up to 1 month) store at 2-8 °C. Long-term, aliquot and store at -28 °C.

Expiry Date: 1 month

Target Details for MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Alternative Name: Microtubule Associated Protein-2 (MAP-2) (2a + 2b)