Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
Specificity
Anti-MAP-2 recognizes (Mr 300 kDa) MAP-2 protein from bovine brain. Strongly cross-reacts with MAP-2 from human, rat, mouse and chicken brains. Anti-MAP-2 (Clone AP20) reacts with the high molecular weight forms (2a & 2b) of MAP-2 but not with the low molecular weight form (2c). Reacts with dendrites and cell bodies of neurons.
Characteristics
MAb to MAP-2 (2a + 2b), Monoclonal antibody to Microtubule Associated Protein-2 (MAP-2) (2a + 2b), Cell culture
Purification
R-phycoerythrin has been covalently conjugated to anti-human CD45RO and chromatographically purified to remove unconjugated antibody and dye, while achieving a fluorochrome/protein (F/P) molar ratio between 0.7-1.3. R-phycoerythrin has a maximum absorbance of 565.5nm. Tissue culture
MAP2
Reactivity: Rat
WB, IHC, ICC, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Can be used in protein blotting and immunohistochemistry, frozen tissues only. Anti-MAP-2 may be used in studies of neuron structure in brain tissue. The suggested working dilution for immunohistochemical staining is approximately 1:500. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid multiple freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. Prepare working dilution only prior to immediate use.
Storage
4 °C/-20 °C
Storage Comment
Short-term (up to 1 month) store at 2-8 °C. Long-term, aliquot and store at -28 °C.