alpha Tubulin antibody (Biotin)

Catalog No. ABIN2380025

The Rabbit Polyclonal anti-alpha Tubulin antibody has been validated for WB, ELISA and IHC. It is suitable to detect alpha Tubulin in samples from Human.

Quick Overview for alpha Tubulin antibody (Biotin) (ABIN2380025)

Target: alpha Tubulin (TUBA1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Tubulin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-alpha Tubulin Antibody (Biotin)

Cross-Reactivity: Cow (Bovine), Chicken, Goat, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Cross-Reactivity (Details): Calculated cross reactivity: Bo Ch Gt Hu Mo Rt Sh

Characteristics: Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1) (Biotin)

Purification: Purified by immunoaffinity chromatography.

Immunogen: Synthetic peptide corresponding to aa 425-451 at the C-Terminal of human alpha tubulin.

Isotype: IgG

Application Details

Application Notes: ELISA: 1:20,000-1:100,000
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterile dH2O.

Buffer: Supplied as a lyophilized powder in PBS, 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10 mg/mL BSA, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: Tubulin, alpha

Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (A and B-tubulin). A and B-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric a-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated A-tubulin is referred to as G-tubulin. Another post-translational modification of detyrosinated a A tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This makes an excellent loading control.

Pathways: Microtubule Dynamics