CD73 antibody (AA 27-252)
Quick Overview for CD73 antibody (AA 27-252) (ABIN238367)
Target
See all CD73 (NT5E) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- AA 27-252
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Specificity
- This antibody is specific for NT5E/CD73.
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Cross-Reactivity (Details)
- Species reactivity (tested):Human.
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Characteristics
- Synonyms: NT5, NTE, NT5E, 5'-nucleotidase
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Purification
- Protein-G affinity chromatography
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Immunogen
- Recombinant Human CD73 (aa 27-252) purified from E. coli
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Isotype
- IgG2b
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anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 520-550), (C-Term) antibody
NT5E Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal RB02846 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibodyNT5E Reactivity: Human WB, IHC, ELISA Host: Mouse Monoclonal 4G6B10 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibodyNT5E Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E Reactivity: Human WB, IHC, FACS, IF, StM Host: Mouse Monoclonal NT5E-2503 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E Reactivity: Human WB, IHC, FACS, IF, ELISA, StM, Coat Host: Mouse Monoclonal NT5E-2545 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 290-550) antibodyNT5E Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibodyNT5E Reactivity: Human WB, IHC, FACS, ELISA Host: Mouse Monoclonal 4G6E3 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 48-527) antibodyNT5E Reactivity: Cow WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody (PE)NT5E Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal AD2 PE
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 151-250) antibodyNT5E Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep WB, FACS, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated
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Application Notes
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ELISA. Western blot: 1/1,000 - 1/2,000. Immunohistochemistry on Paraffin Sections: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
- PBS, pH 7.4 with 0.09 % Sodium Azide as preservative
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C/-20 °C
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Storage Comment
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
Expiry Date
- 12 months
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- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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Alternative Name
- CD73
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Background
- CD73 (Ecto-5'-nucleotidase, 5'-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.Synonyms: 5'-nucleotidase, NT5, NT5E, NTE
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UniProt
- P21589
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Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
Target
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