Clathrin Heavy Chain (CLTC) (Heavy Chain) antibody

Catalog No. ABIN238388

The Mouse Monoclonal anti- antibody has been validated for WB, IF, FACS, IP and EIA. It is suitable to detect in samples from Human, Mouse, Rat, Cow and Pig.

Quick Overview for Clathrin Heavy Chain (CLTC) (Heavy Chain) antibody (ABIN238388)

Target: Clathrin Heavy Chain (CLTC)

Reactivity: Human, Mouse, Rat, Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: BF-06

Product Details

Binding Specificity: Heavy Chain

Specificity: The antibody recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa protein involved in receptor-mediated endocytosis.

Characteristics: Synonyms: CLTC, CLH17, CLTCL2, KIAA0034, Membrane Vesicle Marker, Clathrin heavy chain onchromosome 17

Purification: Ion exchange chromatography

Purity: > 95 % (by SDS-PAGE)

Isotype: IgM

Application Details

Application Notes: Flow Cytometry: 2-5 μg/mL. Positive control: human blood leukocytes. Immunoprecipitation. Western Blotting. Immunocytochemistry. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody at 2 - 8 °C. Do not freeze.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: Clathrin Heavy Chain (CLTC)

Alternative Name: Clathrin Heavy Chain

Background: Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.Synonyms: CLH17, CLTC, CLTCL2, Clathrin heavy chain on chromosome 17, KIAA0034, Membrane Vesicle Marker

Gene ID: 1213

UniProt: Q00610