CD4 antibody

Catalog No. ABIN238451

The Mouse Monoclonal anti-CD4 antibody has been validated for FACS and IP. It is suitable to detect CD4 in samples from Human.

Quick Overview for CD4 antibody (ABIN238451)

Target: CD4

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: MEM-16

Product Details anti-CD4 Antibody

Specificity: The antibody MEM-16 recognizes an epitope EF loop of D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes.

Characteristics: Synonyms: T4/Leu-3, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

Purification: Precipitation methods and size-exclusion chromatography.

Immunogen: HPB cell line (human peripheral blood leukemia T-cells).

Isotype: IgM

Application Details

Application Notes: Flow Cytometry: 10 μg/mLPositive control: Peripheral blood lymphocytesImmunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin Intracellular ligands: p56Lck CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene ID: 920

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events