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CD300a antibody

CD300A Reactivity: Human WB, FACS Host: Mouse Monoclonal MEM-260 unconjugated
Catalog No. ABIN238465
  • Target See all CD300a (CD300A) Antibodies
    CD300a (CD300A)
    Reactivity
    • 35
    • 7
    • 1
    Human
    Host
    • 24
    • 11
    • 5
    Mouse
    Clonality
    • 26
    • 14
    Monoclonal
    Conjugate
    • 17
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD300a antibody is un-conjugated
    Application
    • 17
    • 16
    • 6
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS)
    Specificity
    This antibody reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25 % of circulating T cells and 15 % of circulating B cells.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human
    Characteristics
    Synonyms: CLM-8, CMRF35-H, CMRF35-H9, CMRF-35-H9, IRC1/IRC2, IRp60, CMRF35-like molecule 8,Inhibitory receptor protein 60, NK inhibitory receptor, CD300 antigen-like family member A
    Purification
    Protein A affinity chromatography.
    Immunogen
    HPB human acute lymphoid leukemia cell line.
    Clone
    MEM-260
    Isotype
    IgG1
    Top Product
    Discover our top product CD300A Primary Antibody
  • Application Notes
    Flow Cytometry. Western Blotting (Non-reducing conditions): Positive material: HPB cell lineNegative material: JURKAT human cell line, RAJI human cell line. Sample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing SDS-PAGE sample buffer.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, pH 7.4 containing 15 mM sodium azide as preservative
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    CD300a (CD300A)
    Alternative Name
    CD300A / CMRF35H (CD300A Products)
    Synonyms
    CD300L-B1 antibody, CD300A antibody, clm8 antibody, B230315M08Rik antibody, Clm8 antibody, LMIR1 antibody, MAIR-I antibody, MAIR-Ia antibody, MMAC8 antibody, Pigr4 antibody, mcpir1 antibody, CLM-8 antibody, CMRF-35-H9 antibody, CMRF-35H antibody, CMRF35-H antibody, CMRF35-H9 antibody, CMRF35H antibody, CMRF35H9 antibody, IGSF12 antibody, IRC1 antibody, IRC1/IRC2 antibody, IRC2 antibody, IRp60 antibody, CD300 molecule like family member g antibody, CD300a molecule antibody, CMRF35-like molecule 8 antibody, CD300A molecule antibody, Cd300a molecule antibody, CD300LG antibody, CD300A antibody, LOC483289 antibody, clm8 antibody, Cd300a antibody
    Background
    CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.Synonyms: CD300 antigen-like family member A, CLM-8, CMRF-35-H9, CMRF35-H, CMRF35-H9, CMRF35-like molecule 8, IRC1/IRC2, IRp60, Inhibitory receptor protein 60, NK inhibitory receptor
    Gene ID
    11314
    UniProt
    Q9UGN4
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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