S100A8
Reactivity: Human, Mouse
ELISA, FACS, IF (cc), IF (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Flow cytometry. ELISA. Dot blots. Immunohistochemistry on acetone-fixed frozen sections at 1-2 μg/mL (1: 200 - 1: 100) andparaffin sections at 4 μg/mL (1: 50), pretreatment is not necessary. Suggested positive control: Human tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.89 mg/mL
Buffer
PBS, pH 7.2 with 0.05 % sodium azide
Preservative
Sodium azide
Precaution of Use
Do Not Use Sodium Azide as preservative.
Handling Advice
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage
4 °C/-20 °C
Storage Comment
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Target
S100A8
(S100 Calcium Binding Protein A8 (S100A8))
Alternative Name
S100A8 / Calgranulin-A / MRP8
Background
Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A