IL12B antibody (Internal Region)

Catalog No. ABIN238549

This Goat Polyclonal antibody specifically detects IL12B in WB and ELISA. It exhibits reactivity toward Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for IL12B antibody (Internal Region) (ABIN238549)

Target: IL12B (Interleukin 12 beta (IL12B))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IL12B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-IL12B Antibody

Binding Specificity: Internal Region

Purpose: Il12b / Il12p40 (mouse)

Sequence: EARQQNKYEN YS

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-EARQQNKYENYS

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 38 kDa band observed in Mouse and Rat Skin lysates (calculated MW of 38.2 kDa according to Mouse NP_032378.1 and 38.4 kDa according to Rat NP_072133.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-IL12B antibody (ABIN238549)

Gui, Xiong, Yang, Li, Huang: "Effects of acupuncture on LIF and IL-12 in rats of implantation failure." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 67, Issue 5, pp. 383-90, (2012) (PubMed).

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: l12b

Background: IL12B, Il12p40, interleukin 12B, RP23-388G23.1, Il-12b, Il-12p40, p40, IL-12 p40, IL-23 subunit p40

Gene ID: 16160, 64546

NCBI Accession: NP_032378

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation