ADORA1 antibody (C-Term)

Catalog No. ABIN238564

This Goat Polyclonal antibody specifically detects ADORA1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for ADORA1 antibody (C-Term) (ABIN238564)

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ADORA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-ADORA1 Antibody

Binding Specificity: C-Term

Purpose: Adenosine A1 receptor

Sequence: NDHFRCQPAP PIDED

Specificity: This antibody is expected to recognise isoforms 1, 2 and 3.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence NDHFRCQPAPPIDED, from the C Terminus of the protein sequence according to NP_000665.1, NP_001041695.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 35 kDa band observed in lysates of cell line HEK293 (calculated MW of 36.5 kDa according to NP_000665.1). Recommended concentration:0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ADORA1

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Alternative Name: ADORA1

Background: ADORA1, adenosine A1 receptor, RDC7

Gene ID: 134, 11539, 29290

NCBI Accession: NP_000665, NP_001351994, NP_001351995

Pathways: EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Synaptic Membrane