CLEC16A antibody (Internal Region)

Catalog No. ABIN238572

The Goat Polyclonal anti-CLEC16A antibody (ABIN238572) specifically detects CLEC16A in ELISA, FACS and IF. The antibody is reactive with Human samples.

Quick Overview for CLEC16A antibody (Internal Region) (ABIN238572)

Target: CLEC16A (C-Type Lectin Domain Family 16, Member A (CLEC16A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CLEC16A antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-CLEC16A Antibody

Binding Specificity: Internal Region

Purpose: CLEC16A

Sequence: SLENQDKGGE RP

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SLENQDKGGERP, from the internal region of the protein sequence according to NP_056041.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 70 kDa band in Rat Testis lysates after 0.5 μg/mL antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please no

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and weak expression seen in the cytoplasm of A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cell

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CLEC16A

Target: CLEC16A (C-Type Lectin Domain Family 16, Member A (CLEC16A))

Alternative Name: CLEC16A

Background: CLEC16A, C-type lectin domain family 16, member A, Gop-1, KIAA0350, MGC111457

Gene ID: 23274, 74374

NCBI Accession: NP_056041, NP_001230332