GCNT3 antibody (AA 273-284)

Catalog No. ABIN238637

There is 1 publication for this product available. The Goat Polyclonal anti-GCNT3 antibody is suitable to detect GCNT3 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for GCNT3 antibody (AA 273-284) (ABIN238637)

Target: GCNT3 (Glucosaminyl (N-Acetyl) Transferase 3, Mucin Type (GCNT3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GCNT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-GCNT3 Antibody

Binding Specificity: AA 273-284

Purpose: C2GnT-M (aa 273 to 284)

Sequence: RDTLHLTNKK KD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RDTLHLTNKKKD, from the internal region of the protein sequence according to NP_004742.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 50 kDa band observed in Human Lymph Node lysates (calculated MW of 50.9 kDa according to NP_004742.1). In transfected HEK293 transiently expressing GCNT3 a band of approx. 55 kDa is observed. This band is not observed in the non-transf

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-GCNT3 antibody (ABIN238637)

Petrosyan, Ali, Verma, Cheng, Cheng: "Non-muscle myosin IIA transports a Golgi glycosyltransferase to the endoplasmic reticulum by binding to its cytoplasmic tail." in: The international journal of biochemistry & cell biology, Vol. 44, Issue 7, pp. 1153-65, (2012) (PubMed).

Target Details for GCNT3

Target: GCNT3 (Glucosaminyl (N-Acetyl) Transferase 3, Mucin Type (GCNT3))

Alternative Name: GCNT3

Background: GCNT3, glucosaminyl (N-acetyl) transferase 3, mucin type, C2/4GnT, C2GnT-M, C2GnT2, GnT-M, beta1,6-N-acetylglucosaminyltransferase

Gene ID: 9245

NCBI Accession: NP_004742

Pathways: Production of Molecular Mediator of Immune Response