FZD7 antibody (C-Term)

Catalog No. ABIN238677

This anti-FZD7 antibody is a Goat Polyclonal antibody detecting FZD7 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for FZD7 antibody (C-Term) (ABIN238677)

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FZD7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-FZD7 Antibody

Binding Specificity: C-Term

Purpose: Frizzled 7

Sequence: RFYHRLSHSS KGET

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RFYHRLSHSSKGET, from the C Terminus of the protein sequence according to NP_003498.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60 kDa band observed in lysates of cell lines HEK293 and HepG2 (calculated MW of 63.6 kDa according to NP_003498.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FZD7

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Alternative Name: FZD7

Background: Rizzled 7, frizzled homolog 7 (Drosophila), FZD7, FzE3, Frizzled, drosophila, homolog of, 7

Gene ID: 8324, 14369

NCBI Accession: NP_003498

Pathways: WNT Signaling, Stem Cell Maintenance