FZD4 antibody (Internal Region)

Catalog No. ABIN238678

This Goat Polyclonal antibody specifically detects FZD4 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for FZD4 antibody (Internal Region) (ABIN238678)

Target: FZD4 (Frizzled Family Receptor 4 (FZD4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FZD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-FZD4 Antibody

Binding Specificity: Internal Region

Purpose: Frizzled 4

Sequence: DEEERRCDPI RIS

Cross-Reactivity: Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DEEERRCDPIRIS, from the internal region of the protein sequence according to NP_036325.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in Human Colon and Small Intestine (Ileum) lysates (calculated MW of 59.9 kDa according to NP_036325.2). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FZD4

Target: FZD4 (Frizzled Family Receptor 4 (FZD4))

Alternative Name: FZD4

Background: Frizzled 4, frizzled homolog 4 (Drosophila), FZD4, EVR1, FEVR, FZD4S, Fz-4, FzE4, GPCR, MGC34390, WNT receptor frizzled-4, exudative vitreoretinopathy 1 (autosomal dominant Criswick-Schepens syndrome), frizzled 4

Gene ID: 8322, 64558

NCBI Accession: NP_036325

Pathways: WNT Signaling, Hormone Transport, Sensory Perception of Sound