RPE65 antibody (Internal Region)

Catalog No. ABIN238680

The Goat Polyclonal anti-RPE65 antibody (ABIN238680) specifically detects RPE65 in WB and ELISA. The antibody is reactive with Rat samples.

Quick Overview for RPE65 antibody (Internal Region) (ABIN238680)

Target: RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This RPE65 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-RPE65 Antibody

Binding Specificity: Internal Region

Purpose: RPE65

Sequence: EVKKNARKAP QPE

Cross-Reactivity: Dog, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-EVKKNARKAPQPE

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 65 kDa band observed in Rat Retina lysates (calculated MW of 60.9 kDa according to Human NP_000320.1 and 61.0 kDa according to Rat NP_446014.1). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RPE65

Target: RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))

Alternative Name: RPE65

Background: MRPE65, sRPE65, retinal pigment epithelium-specific protein 65 kDa, LCA2, RP20, rd12, RBP-binding membrane protein, p63, retinal pigment epithelium-specific 61 kDa protein, retinal pigment epithelium-specific protein (65kD), retinitis pigmentosa 20

Gene ID: 6121

NCBI Accession: NP_000320