CD74 antibody

Catalog No. ABIN241561

This Mouse Monoclonal antibody specifically detects CD74 in FACS, IHC, IP, IHC (p) and IHC (fro). It exhibits reactivity toward Human and Baboon.

Quick Overview for CD74 antibody (ABIN241561)

Target: CD74 (HLA-DR-gamma (CD74))

Reactivity: Human, Baboon

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD74 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: LN2

Product Details anti-CD74 Antibody

Specificity: SU-DHL-4 cells

Purification: Affinity purified

Immunogen: Cells.

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Flo (1 μg/10 x10⁶), IHC, IHC-Fr, IHC-P (10 μg/mL), IP

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Phosphate-buffered solution,  pH 7.2, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 4°C. Store undiluted.

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74 / CLIP

Background: Name/Gene ID: CD74

Synonyms: CD74, CD74 antigen, DHLAG, HLADG, HLA-DR-gamma, Ia-associated invariant chain, p33, MHC HLA-DR gamma chain, Ia-GAMMA

Gene ID: 972

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints