MSH2 antibody (AA 550-600)

Catalog No. ABIN241571

The Rabbit Polyclonal anti-MSH2 antibody is suitable to detect MSH2 in samples from Human and Mouse. It has been validated for WB, IHC and IHC (p).

Quick Overview for MSH2 antibody (AA 550-600) (ABIN241571)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MSH2 Antibody

Binding Specificity: AA 550-600

Specificity: The amino acid sequence used as immunogen is 100 % homologous in human, dog and S. cerevisiae, 94 % in mouse and rat, and 76 % in zebrafish.

Purification: Protein G purified

Immunogen: A portion of amino acids 550-600 of human MSH2.

Type of Immunogen: Protein fragment

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Alternative Name: MSH2

Background: Name/Gene ID: MSH2

Synonyms: MSH2, COCA1, FCC1, HNPCC, HNPCC1, MutS protein homolog 2, HMSH2, LCFS2

Gene ID: 4436

UniProt: P43246

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response